What is the function of DNA ligase in DNA replication?

DNA ligase plays a critical role in DNA replication by joining two DNA fragments through the formation of a phosphodiester bond. This is particularly important during the replication of the lagging strand, where DNA is synthesized in short segments known as Okazaki fragments. After DNA polymerase synthesizes these fragments, ligase comes in to connect them, ensuring that the DNA strand is continuous.

The action of DNA ligase is essential for maintaining the integrity of the new DNA molecules formed during replication. By sealing the gaps between these DNA fragments, ligase helps to complete the replication process and contributes to the overall stability of the genetic material.