Which technique produces a genetically engineered organism with one non-functional gene?

Gene knockout (KO) is a technique specifically designed to create genetically engineered organisms by disabling or "knocking out" a specific gene. This is typically achieved through methods such as homologous recombination, CRISPR-Cas9, or other genome editing techniques that introduce mutations or deletions, effectively rendering the targeted gene non-functional.

In the context of creating an organism with one non-functional gene, the knockout approach is particularly effective because it allows researchers to study the effects of the absence of that gene on the organism's phenotypic traits and biological processes. This provides valuable insights into the gene’s function and its role in the organism, making KO a fundamental method in genetic research, functional genomics, and the development of model organisms.

Other options, such as genome editing, gene expression, and gene pool, serve different biological purposes. Genome editing is broader and can involve both knocking out genes and inserting or modifying genes to achieve desired traits. Gene expression refers to the processes that use the information encoded in genes to produce functional gene products, such as proteins, and does not inherently involve the creation of non-functional genes. Finally, a gene pool refers to the total collection of genes within a population, which does not directly pertain to the manipulation